A promising α-glucosidase and α-amylase inhibitors based on benzimidazole-oxadiazole hybrid analogues: Evidence based in vitro and in silico studies
Hayat Ullah, Imad Uddin, Hafeeza Zafar Ali, Wagma Hassan, Gul Mehnaz, Laiba Maryam, Maliha Sarfraz, Muhammad Saleem Khan, Mohammad Shahidul Islam, Zainab M. Almarhoon, Rashid Iqbal, Muhammad Nabi
Abstract
The present study reports the design and synthesis of new benzimidazole-oxadiazole compounds as potent inhibitors of α-glucosidase and α-amylase. The synthesized molecules were characterized through different techniques such as 1 HNMR, 13 CNMR, HREI-MS and evaluated for their in vitro inhibitory activities against these enzymes. Among the compounds screened, compound 8 demonstrated the highest inhibitory activity against both α -glucosidase (IC 50 = 11.60 µM) and α -amylase (IC 50 = 6.20 µM). Molecular docking analyses were conducted to investigate the binding modes and interactions of the active compounds within the enzyme active sites. The results demonstrate that several benzimidazole-oxadiazole hybrids exhibited potent inhibitory effects on both α-glucosidase and α-amylase, suggesting their promise as antidiabetic agents .