A Dual Anchoring Strategy for the Directed Evolution of Improved Artificial Transfer Hydrogenases Based on Carbonic Anhydrase
Alina Stein, Dongping Chen, Nico V. Igareta, Yoann Cotelle, Johannes G. Rebelein, Thomas R. Ward
Abstract
) and >350 turnovers on a preparative scale). To evaluate the substrate scope, the best hits of each generation were tested with eight substrates. X-ray analysis, carried out at various stages of the evolutionary trajectory, was used to scrutinize (i) the nature of the covalent linkage between the cofactor and the host as well as (ii) the remodeling of the substrate-binding pocket.
Topics & Concepts
ChemistryCofactorCovalent bondCombinatorial chemistryCarbonic anhydraseEnantioselective synthesisLigand (biochemistry)SelectivityStereochemistryCatalysisEnzymeBiochemistryOrganic chemistryReceptorCyclopropane Reaction MechanismsEnzyme Catalysis and ImmobilizationEnzyme function and inhibition