Investigation and improvement of catalytic activity of G-quadruplex/hemin DNAzymes using designed terminal G-tetrads with deoxyadenosine caps
Yanwei Cao, Pi Ding, Luyan Yang, Wenjing Li, Yu Luo, Jine Wang, Renjun Pei
Abstract
A. Our data demonstrated that 3' dA caps are versatile residues in promoting the interaction of G4s with hemin. Thus, by increasing the number of 3' dA caps, the DNAzyme of 3'A5'-5'GG3'-3'GG5'-5'A3' with two 5'-terminal G-tetrads can exhibit significantly high catalytic activity, which is comparable to that of 5'A3'-3'GG5'-5'GG3'-3'A5' with two 3'-terminal G-tetrads. This study may provide insights into the catalytic mechanism of G4-based DNAzymes and strategies for promoting their catalytic activities.
Topics & Concepts
DeoxyribozymeG-quadruplexHeminDeoxyadenosineCatalysisChemistrySteric effectsPeroxidaseTerminal (telecommunication)StereochemistryDNACombinatorial chemistryBiochemistryEnzymeComputer scienceHemeTelecommunicationsAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryRNA Interference and Gene Delivery