Macrophage Polarization Related to Biomimetic Calcium Phosphate Coatings: A Preliminary Study
Jiping Chen, Yiwen Zhou, Xingnan Lin, Li Huang
Abstract
Biomimetic calcium phosphate (BioCaP) coatings were used to deliver bone morphogenetic protein 2 (BMP2), and enhance osteogenesis. However, the mechanism for BioCaP coatings interacting with the immune response during bone regeneration remains unclear. In this preliminary study, the effect of BioCaP coatings on macrophage polarization without (BioCaP group) or with BMP2 (BioCaP+Inc.BMP2 group) was investigated. RAW 264.7 cells were cultured on the rough and platelike surfaces of coatings in BioCaP and BioCaP+Inc.BMP2 groups, while cultured on smooth surfaces in the group without material for 5 days. The BioCaP coatings per se modulated the switch of M1 to M2 phenotype from day 3, which promoted the expressions of Arg1 and CD 206 but reduced the expression of TNF-α compared with No material group. To detect the microenvironmental changes, the concentrations of calcium ion (Ca2+) and inorganic phosphate (Pi), pH values, as well as calcium phosphate crystal pattern were examined. The trends of ionic environmental changes were closely related with macrophage phenotype switch. These results suggest that BioCaP coating itself may affect the macrophage polarization through surface topography, surrounding ionic environment and calcium phosphate crystal pattern.