MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway
María Otilia Delgadillo, Guillermo Ruano, Jan Zouhar, Michael Sauer, Jinbo Shen, Aleksandra Lazarova, Maite Sanmartín, Louis Tung Faat Lai, Cesi Deng, Pengwei Wang, Patrick J. Hussey, José Juan Sánchez‐Serrano, Liwen Jiang, Enrique Rojo
Abstract
-Golgi network (TGN) and the multivesicular bodies (MVBs), supporting that the trafficking step between these compartments is essential for segregating vacuolar proteins from those destined for secretion. Importantly, the GARP tethering complex subunits MTV16/VPS51 and MTV17/VPS54 were found at endoplasmic reticulum (ER)- and microtubule-associated compartments (EMACs). Moreover, MTV16/VPS51 interacts with the motor domain of kinesins, suggesting that, in addition to tethering vesicles, the GARP complex may regulate the motors that transport them. Our findings unveil a previously uncharacterized compartment of the plant vacuolar trafficking pathway and support a role for microtubules and kinesins in GARP-dependent transport of soluble vacuolar cargo in plants.