Co-immobilized bienzyme of horseradish peroxidase and glucose oxidase on dopamine-modified cellulose–chitosan composite beads as a high-efficiency biocatalyst for degradation of acridine
Yaohua Gu, Lin Yuan, Mingming Li, Xinyu Wang, Deyu Rao, Xiaoyan Bai, Keren Shi, Haiming Xu, Shaozhang Hou, Huiqin Yao
Abstract
covalent binding, designated as Ce-Cs@DA/HRP-GOD beads, and found that the bienzyme biocatalyst had a good ability to catalytically degrade acridine in wastewater. SEM, EPR, FTIR, and XRD were used to characterise the structure and properties of the Ce-Cs@DA/HRP-GOD beads. The co-immobilized bienzyme biocatalyst with a small amount of HRP exhibited better degradation efficiency for acridine (99.5%, 8 h) in simulated wastewater compared to the Ce-Cs@DA/HRP (93.8%, 8 h) and Ce-Cs@DA/GOD (15.8%, 8 h) beads alone. In addition, a reusability study showed that the co-immobilized bienzyme biocatalyst maintained a degradation rate of 61.2% after six cycles of acridine degradation. The good biodegradability and reusability of the biocatalyst might be due to the synergistic effect of bienzyme HRP-GOD, including the strong covalent bonding. Accordingly, the co-immobilized bienzyme biocatalyst based on the cascade reaction may pave the way for efficient and eco-friendly treatment of industrial wastewater.