Unmixing for ultra-high-plex fluorescence imaging
Archibald Enninful, Alev Baysoy, Rong Fan
Abstract
Writing in Nature communications, Seo and collaborators presented PICASSO as a method to achieve 15-color imaging of spatially overlapping proteins using no reference emission spectra in a single staining and imaging round. This accessible tool has the potential to be applied to diverse applications within the spatial biology field without neglecting accuracy.
Topics & Concepts
PICASSOComputer scienceFluorescenceComputational biologyArtificial intelligencePhysicsOpticsBiologyArtArt historyPaintingCell Image Analysis TechniquesAdvanced Fluorescence Microscopy TechniquesAdvanced Biosensing Techniques and Applications