The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi
Clara Woodcraft, Yit‐Heng Chooi, Indra Roux
Abstract
CRISPR/Cas technologies has opened up avenues for the manipulation of biosynthetic gene clusters across diverse filamentous fungi. This review discusses the innovative and diverse strategies for fungal natural product discovery and engineering made possible by CRISPR/Cas-based tools. We also provide a guide into multiple angles of CRISPR/Cas experiment design, and discuss current gaps in genetic tool development for filamentous fungi and the promising opportunities for natural product research.
Topics & Concepts
CRISPRToolboxNatural productComputational biologyGene engineeringBiologyGene clusterGeneComputer scienceGeneticsProgramming languageBiochemistryRecombinant DNACRISPR and Genetic EngineeringInsect symbiosis and bacterial influencesMicrobial Metabolism and Applications