PARP1-DNA co-condensation: the driver of broken DNA repair
Xiang Wei, Fangfang Zhou, Long Zhang
Abstract
DNA double-strand break (DSB) sites that prevent the disjunction of broken DNA ends are formed through poly (ADP-ribose) (PAR) polymerase 1 (PARP1)-DNA co-condensation. The co-condensates apply mechanical forces to hold the DNA ends together and generate enzymatic activity for the synthesis of PAR. PARylation can promote the release of PARP1 from DNA ends and recruit various proteins, such as Fused in sarcoma (FUS) proteins, thereby stabilizing broken DNA ends and preventing their separation.
Topics & Concepts
DNADNA repairChemistryComputational biologyBiologyMolecular biologyGeneticsMedicinePARP inhibition in cancer therapyDNA Repair MechanismsCRISPR and Genetic Engineering