Triazine-pyridine chemistry for protein labelling on tyrosine
Hongfei Jiang, Qing Zhang, Yue Zhang, Huxin Feng, Hao Jiang, Fan Pu, Rilei Yu, Zheng Zhong, Chaoming Wang, Yi Man Eva Fung, Pilar Blasco, Yong‐Xin Li, Tao Jiang, Xuechen Li
Abstract
Herein, we discover the new reactivity of the 1,3,5-triazine moiety reacting with a phenol group and report the development of biocompatible and catalyst-free triazine-pyridine chemistry (TPC) for tyrosine labelling under physiological conditions and profiling in the whole proteome. TPC exhibited high tyrosine chemoselectivity in biological systems after cysteine blocking, displayed potential in tyrosine-guided protein labelling, and had bio-compatibility in live cells.
Topics & Concepts
LabellingChemistryTyrosineMoietyChemoselectivityTriazineCysteineProteomeBiochemistryCombinatorial chemistryOrganic chemistryCatalysisEnzymeClick Chemistry and ApplicationsChemical Synthesis and AnalysisMonoclonal and Polyclonal Antibodies Research