Modulating the pH profile of the pullulanase from Pyrococcus yayanosii CH1 by synergistically engineering the active center and surface
Ting Xie, Li Zhou, Laichuang Han, Wenjing Cui, Zhongmei Liu, Zhongyi Cheng, Junling Guo, Zhemin Zhou
Topics & Concepts
ThermostabilityPullulanaseHydrolysisActive centerChemistryActive siteProtein engineeringHistidineSurface engineeringHydrolaseCatalysisDirected evolutionMutantEnzymeCombinatorial chemistryBiochemistryMaterials scienceNanotechnologyGeneEnzyme Production and CharacterizationStudies on Chitinases and ChitosanasesPhytase and its Applications