A fluorescence-based genetic screen reveals diverse mechanisms silencing small RNA signaling in <i>E. coli</i>
Jiandong Chen, Leann To, François De Mets, Xing Luo, Nadim Majdalani, Chin‐Hsien Tai, Susan Gottesman
Abstract
-acetyltransferase (GNAT). Two specific regulators were identified. AspX, a 3'end-derived small RNA, specifically represses RyhB signaling via an RNA sponging mechanism. YicC, a previously uncharacterized but widely conserved protein, triggers rapid RyhB degradation via collaboration with the exoribonuclease PNPase. These findings greatly expand our knowledge of regulation of bacterial sRNA signaling and suggest complex regulatory networks for controlling iron homeostasis in bacteria. The fluorescence-based genetic screen system described here is a powerful tool expected to accelerate the discovery of novel regulators of sRNA signaling in many bacteria.