Genetic Characterization and Passage Instability of a Hybrid Plasmid Co-Harboring <i>bla</i> <sub>IMP-4</sub> and <i>bla</i> <sub>NDM-1</sub> Reveal the Contribution of Insertion Sequences During Plasmid Formation and Evolution
Xi Li, Jintao He, Yan Jiang, Minfei Peng, Yunsong Yu, Ying Fu
Abstract
In this study, we characterized a novel plasmid from a carbapenem-resistant K. michiganensis (CRKM) isolate, which harbors two metallo-β-lactamases (MBLs), IMP-4 and NDM-1, is capable of transconjugation and contains three replicons. Our results first expand the diversity of plasmids co-harboring carbapenemase genes in Enterobacterales , which exhibits epidemic importance in bacterial resistance.
Topics & Concepts
PlasmidBiologyGeneticsGeneSouthern blotInsertion sequenceSequence analysisDNAMolecular biologyPlasmid preparationT-DNA Binary systemPolymerase chain reactionDNA sequencingExperimental evolutionRestriction enzymeSequence (biology)Mobile genetic elementsRestriction mapAntibiotic Resistance in BacteriaBacterial biofilms and quorum sensingAntimicrobial agents and applications