Accurate, Sensitive, and Rapid Detection of Pseudomonas aeruginosa Based on CRISPR/Cas12b with One Fluid-Handling Step
Xiaotong Qiu, Xueping Liu, Ruixue Wang, Xiao Ma, Lichao Han, Jiang Yao, Zhenjun Li
Abstract
This study reports a P. aeruginosa CRISPR-top assay which can precisely identify P. aeruginosa using nucleic acids from pure cultures or clinical samples in one pot with one fluid-handling step. The P. aeruginosa CRISPR-top reaction is suitable for on-site testing, and its diagnostic performance can be compared with that of qPCR.
Topics & Concepts
Pseudomonas aeruginosaPrimer (cosmetics)CRISPRLoop-mediated isothermal amplificationSputumMicrobiologyPrimer extensionDetection limitBiologyGold standard (test)BacteriaDNAChemistryMedicineChromatographyGeneGeneticsBase sequenceInternal medicinePathologyTuberculosisOrganic chemistryCRISPR and Genetic EngineeringBiosensors and Analytical DetectionBacterial biofilms and quorum sensing