Diselenides as novel effective fluorescence quenchers to construct a two-photon fluorescent probe for thiols in a mouse stroke model
Tao Ma, Hao Gao, Jun Wu, Jintao Zhao, Bingbing Chang, Zhibin Zhang, Shengxiang Zhang, Baoxin Zhang, Jianguo Fang
Abstract
A fluorescence quenching mechanism using linear diselenides was proposed for the first time through a combination of intramolecular charge transfer (ICT) and Förster resonance energy transfer (FRET). Herein, we synthesized and screened a two-photon fluorescent probe AFC-SeSe, demonstrating a remarkable 300-fold increase in response to glutathione (GSH). Additionally, AFC-SeSe enabled real-time observation of increased thiol levels following treatment within a short timeframe in a mouse model of stroke.
Topics & Concepts
Förster resonance energy transferFluorescenceIntramolecular forcePhotochemistryQuenching (fluorescence)ChemistryEnergy transferFluorescence in the life sciencesBiophysicsChemical physicsOpticsStereochemistryPhysicsBiologySulfur Compounds in BiologySulfur-Based Synthesis TechniquesOrganoselenium and organotellurium chemistry