Electrochemiluminescence sensor based on resonance energy transfer for detection of chlortetracycline hydrochloride in food
Yujia Song, Li Tian, Ruidan Li, Yanjia Guo, Pengfei Han, Hanyue Jiang, Wenzhuo Wang, Jing Wang
Abstract
In this work, a highly sensitive electrochemiluminescence (ECL) sensor for Chlortetracycline hydrochloride (CTC) detection was constructed. The complex of g-C 3 N 4 -chitosan (g-C 3 N 4 @CS) and Pt NPs@MIL-101(Fe) were modified to the electrode. In g-C 3 N 4 -S 2 O 8 2− system, g-C 3 N 4 served as an ECL signal. Synthesized Pt NPs@MIL-101(Fe) acted as a novel coreaction promoter of the system, further enhancing the ECL signal. With the addition of CTC, the ECL signal decreased, and this quenching phenomenon was mainly because of ECL-RET and the related mechanism was studied. Under the best conditions, a strong linear relationship was observed between the change in ECL signal (ΔI) and the logarithm of CTC concentration (lgC CTC ), spanning from 5 × 10 −14 to 5 × 10 −9 mol L −1 . The linear equation was ΔI = 16,817 + 1179.4 lgC CTC with a correlation coefficient of 0.995. The method demonstrates an exceptionally low detection limit of 1.7 × 10 −14 mol L −1 (S/N = 3), making it suitable for real sample analysis.