Construction of an Escherichia coli Strain Lacking Fimbriae by Deleting 64 Genes and Its Application for Efficient Production of Poly(3-Hydroxybutyrate) and <scp>l</scp> -Threonine
Jun Qiao, Xin Tan, Hong‐Yu Ren, Zheng Wu, Xiaoqing Hu, Xiaoyuan Wang
Abstract
In this study, we investigated the interaction between the biosynthesis and assembly of fimbriae and intracellular metabolic networks in E. coli. We found that eliminating fimbriae could effectively improve the production of polyhydroxyalkanoate and l-threonine in E. coli MG1655. These results contribute to understanding the necessity of fimbriae and the advantages of fimbria removal for industrial microorganisms. The knowledge gathered from this study may be applied to the development of superior chassis microorganisms.
Topics & Concepts
FimbriaEscherichia coliThreonineStrain (injury)PolyhydroxyalkanoatesMicrobiologyEnterobacteriaceaeBiosynthesisBiochemistryGeneBiologyChemistryBacteriaGeneticsEnzymeSerineAnatomybiodegradable polymer synthesis and propertiesMicrobial Metabolic Engineering and BioproductionEnzyme Catalysis and Immobilization