Proteomics and Metabolomics Reveal the Regulatory Pathways of Ripening and Quality in Post-Harvest Kiwifruits
Xiaocheng Tian, Lingcheng Zhu, Nanxiang Yang, Jianyu Song, Haiyan Zhao, Jing Zhang, Fengwang Ma, Mingjun Li
Abstract
Understanding the metabolic modulation of major quality traits during ripening is critical for fruit quality improvement in kiwifruits. Here, integrated proteomic and metabolomic profiling was undertaken to comprehensively examine the dynamics of kiwifruit ripening. This data set presents a global view of the critical pathways involved in fruit ripening, and the contributions of key events to the regulation of kiwifruit ripening and softening, amino acid metabolism, balance in sugar accumulation and organic acid metabolism, glycolysis, and tricarboxylic acid (TCA) pathways were discussed. We suggested key enzymes for starch synthesis and degradation, including AGPase, SS, and SBE, especially for BMY, which was considered a key enzyme for starch degradation. In addition, our analysis implicated the key enzymes ACO4 and ACS9 in ethylene synthesis and the aspartate aminotransferase ASP3 in the conversion of amino acids. These results provide new insights into the modulation of fruit ripening, metabolism, and quality in post-harvest kiwifruits.