Simultaneous introduction of multiple biomacromolecules into plant cells using a cell-penetrating peptide nanocarrier
Chonprakun Thagun, Yoko Motoda, T. Kigawa, Yutaka Kodama, Keiji Numata
Abstract
-BP100 as a carrier to deliver multiple biomolecules into plant cells and performed a bimolecular fluorescence complementation assay to assess the simultaneous introduction of multiple biomolecules. We demonstrate that multiple biomolecule/CPP cargos can be simultaneously internalized by a particular plant cell, albeit with different efficiencies. We present a cutting-edge technique for codelivery of multiple biomolecules into plant cells that can be used for elucidation of functional correlations and for metabolic engineering.
Topics & Concepts
NanocarriersCell-penetrating peptidePeptidePlant cellCellNanotechnologyMaterials scienceBiophysicsChemistryBiologyNanoparticleBiochemistryGeneAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliverySilk-based biomaterials and applications