Biochar effect on sheep feed intake, growth rate and ruminant in vitro and in vivo methane production
Vibeke Lind, Özge Sızmaz, Ahu Demirtaş, Mert Sudağıdan, Simon Weldon, Alice Budai, Adam O’Toole, Dejan Miladinovic, G Jörgensen
Abstract
Biochar, which is the product of biomass pyrolysis, has been suggested as a feed supplement to improve performance in livestock systems and reduce greenhouse gas (GHG) emissions. The aim of the current study was to investigate in vitro and in vivo potential of biochar to favourably modify rumen fermentation (e.g., an increase in total Short Chained Fatty Acid (SCFA) concentration and a change in SCFA profile), reduce methane emission and increase sheep growth performance. Four concentrates were produced with biochar inclusion of 0, 10, 23 and 46 g/kg DM. The experimental diets for the in vitro experiments consisted of straw and concentrate in a 60:40 ratio and included measurements of total gas and methane (CH4) production, pH, ammonia nitrogen (NH3-N), SCFA, and microbial assays (total bacteria and methanogenic archaea). Two in vivo experiments were performed where the animals received ad libitum forage with 0.4 kg concentrate daily. Experiment 1 investigated the daily DM intake of sheep while experiment 2 investigated daily growth rate and CH4 emission of lambs. Inclusion of biochar had no impact on in vitro total gas production (ml/200 mg DM substrate) (P = 0.81) and CH4 production (ml/200 mg DM substrate) (P = 0.93). In vitro total SCFA concentration increased (P < 0.05) while acetate to propionate ratio (A:P) tended to decrease (P = 0.05) with both doses of biochar. Total bacteria decreased with the highest biochar inclusion in vitro (P < 0.05). Sheep’s dry matter intake (kg/d) increased when low and medium levels but not when a higher level of biochar was added to the diet (P < 0.001). Inclusion of biochar did not significantly impact lamb’s daily growth rate (g/d) (P = 0.61) or enteric CH4 emissions (g/kg DM) (P = 0.43). We conclude that biochar supplementation had no favourable impacts on in vitro and in vivo CH4 production or on lamb’s growth rate. Further research with well-characterized biochar is needed to gain a better understanding of the potential of biochar as a feed additive for ruminant livestock.