Cu-MOF/Aptamer/Magnetic Bead Sensor for Detection of Breast Cancer Biomarkers HER2 and ER
Xiaofang Liu, Ya Zhang, Ning Li, Zhihao Yang, Siyi Yang, Jiawei Li, Jian Chen, Danqun Huo, Changjun Hou
Abstract
Cancer markers are used for early cancer detection and follow-up monitoring so that patients receive the most appropriate treatment to alleviate suffering and prolong life. Herein, a metal–organic framework (MOF) combined with magnetic beads (MB) is proposed as a nanosensor for detecting breast cancer biomarkers based on magnetic enrichment and separation. With the porous structure of Cu-MOF, a large number of aptamers are adsorbed inside the pore to specifically capture the antigen. Then, Cu-MOF@antigen is enriched and separated by MB modified with the corresponding antibody. Supernatant Cu-MOF and Cu-MOF@antigen@MB are used as colorimetric, fluorescent, and electrochemical sensing elements for the quantitative detection of HER2 and ER. The detection ranges for HER2 and ER are 4.5 fg/mL–20 ng/mL and 10 fg/mL–20 ng/mL, respectively. It also demonstrated good performance in the detection of human serum samples, with recoveries ranging from 89.00 to 107.57%. This nanosensor has promising selectivity, stability, and practical applicability, providing the possibility for its in-depth development.