Litcius/Paper detail

Enhanced synthesis of chloramphenicol intermediate L-threo-p-nitrophenylserine using engineered L-threonine transaldolase and by-product elimination

Zhiwen Xi, Yan Xu, Zhiyong Liu, Xinyi Zhang, Qiang Zhu, Lihong Li, Rongzhen Zhang

2024International Journal of Biological Macromolecules12 citationsDOI

Topics & Concepts

TransaldolaseChemistryDirected evolutionEnantiomeric excessAlcohol dehydrogenaseFormate dehydrogenaseCombinatorial chemistryBiocatalysisStereochemistrySaturated mutagenesisBiochemistryMutantCatalysisEnzymeEnantioselective synthesisFormateReaction mechanismPentose phosphate pathwayGeneGlycolysisEnzyme Catalysis and ImmobilizationAmino Acid Enzymes and MetabolismCarbohydrate Chemistry and Synthesis