Neddylation of Enterovirus 71 VP2 Protein Reduces Its Stability and Restricts Viral Replication
Huiqiang Wang, Ming Zhong, Boming Cui, Haiyan Yan, Shuo Wu, Kun Wang, Yuhuan Li
Abstract
Neddylation is a ubiquitin-like posttranslational modification by conjugation of neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8) to specific proteins for regulation of their metabolism and biological activities. In this study, we demonstrated for the first time that EV71 VP2 protein is neddylated at K69 residue to promote viral protein degradation and consequentially suppress multiplication of the virus. Our findings advance knowledge related to the roles of VP2 in EV71 virulence and the neddylation pathway in the host restriction of EV71 infection.
Topics & Concepts
NeddylationNEDD8BiologyUbiquitinViral replicationDDB1Cell biologyViral proteinPicornavirusVirusBiochemistryUbiquitin ligaseVirologyGeneRNAViral Infections and Immunology ResearchVirus-based gene therapy researchRNA and protein synthesis mechanisms