Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity
Khac Huy Ngo, Renliang Yang, Poulomi Das, Giang K. T. Nguyen, Kah Wai Lim, James P. Tam, Bin Wu, Anh Tuân Phan
Abstract
G-quadruplexes (G4) are non-canonical nucleic acid structures with important implications in biology. Based on an α-helical fragment of the RHAU helicase that displays high specificity for parallel-stranded G-quadrplexes, herein we demonstrate its head-to-tail cyclization by a high-efficiency ligase. The cyclic peptide exhibits superior stability and binding affinity to a G-quadruplex, and can serve as an excellent investigational tool for chemical biology applications.
Topics & Concepts
G-quadruplexChemistryPeptideCombinatorial chemistryProtein stabilityBiophysicsStereochemistryBiochemistryDNABiologyDNA and Nucleic Acid ChemistryChemical Synthesis and AnalysisRNA and protein synthesis mechanisms