Biodegradation of Punicalagin into Ellagic Acid by Selected Probiotic Bacteria: A Study of the Underlying Mechanisms by MS-Based Proteomics
Víctor Caballero, Mario Estévez, Francisco A. Tómas‐Barberán, David Morcuende, Irene Martín, Josué Delgado
Abstract
). Further, we evaluated the molecular mechanism governing this transformation through label-free comparative MS-based proteomics. All tested LAB strains were capable of transforming punicalagin into ellagic acid, while the biosynthesis of urolithins was not observed. Proteomic analysis revealed an increase of generic transglycosylases that might have a hydrolytic role in the target phenolic molecule, coupled with an increase in the quantity of ATP-binding cassette (ABC) transporters, which might play a relevant role in transporting the resulting byproducts in and out of the cell.
Topics & Concepts
Ellagic acidChemistryPunicaLactic acidBiochemistryTandem mass spectrometryBacteriaChromatographyPolyphenolMass spectrometryBiologyAntioxidantGeneticsHorticulturePomegranate: compositions and health benefitsPhytase and its Applications