Immunochromatographic assay for rapid detection of flupyradifurone in grape, blueberry, and tomato samples
Qianqian Lü, Hongliu Ding, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Lingling Guo
Abstract
Flupyradifurone (FPF) is a neonicotinoid insecticide that effectively controls the spread of various pests. In this study, we established an immunochromatographic assay based on a highly specific and sensitive anti-FPF monoclonal antibody (mAb) to screen for FPF residues in grapes, blueberries, and tomatoes. The cut-off value for the immunochromatographic assay was 5 mg/kg for grapes and 10 mg/kg for blueberries and tomatoes. The calculated limit of detection of the immunochromatographic assay was 0.009 mg/kg, 0.033 mg/kg, and 0.040 mg/kg for grapes, blueberries, and tomatoes, respectively. The recovery rates of the immunochromatographic assay were 97.0-108.2 % in grape samples, 90.9-105.1 % in blueberry samples, and 94.0-103.7 % in tomato samples, and the detection results were highly consistent with LC-MS/MS results. Therefore, this immunochromatographic assay was an effective and rapid tool for screening for FPF in grapes, blueberries, and tomatoes.