Single-shot 3D wide-field fluorescence imaging with a Computational Miniature Mesoscope
Yujia Xue, Ian G. Davison, David A. Boas, Lei Tian
Abstract
features a compact lightweight design that integrates a microlens array for imaging and a light-emitting diode array for excitation. Its expanded imaging capability is enabled by computational imaging that augments the optics by algorithms. We experimentally validate the mesoscopic imaging capability on 3D fluorescent samples. We further quantify the effects of scattering and background fluorescence on phantom experiments.
Topics & Concepts
MicroscopeMicrolensLight sheet fluorescence microscopyFluorescence-lifetime imaging microscopyOpticsImaging phantomMaterials scienceMesoscopic physicsMicroscopyFluorescence microscopeField of viewBiophotonicsFluorescenceImage resolutionDepth of fieldBiological imagingResolution (logic)Optical imagingScatteringPreclinical imagingBiological specimenImaging techniqueMolecular imagingComputer scienceDirect imagingMedical imagingImage processingLive cell imagingImaging technologyTemporal resolutionField (mathematics)Computational modelLens (geology)High resolutionComputational photographyDiodeDetectorNanotechnologyLight scatteringImage sensorAdvanced Fluorescence Microscopy TechniquesDigital Holography and MicroscopyNear-Field Optical Microscopy