Ligase detection reaction amplification-activated CRISPR-Cas12a for single-molecule counting of FEN1 in breast cancer tissues
Ziyue Wang, S. S. Teng, Ning-ning Zhao, Yun Han, Dongling Li, Chun‐yang Zhang
Abstract
U. Moreover, it can be employed to screen the FEN1 inhibitors and quantitatively measure the FEN1 activity in human cells and breast cancer tissues, holding great promise in clinical diagnosis and drug discovery.
Topics & Concepts
CRISPRBreast cancerDNA ligaseMolecular biologyChemistryDNACancerBiologyBiochemistryGeneticsGeneCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesSingle-cell and spatial transcriptomics