Paper-Based Biosensors for the Detection of Nucleic Acids from Pathogens
Jiangshan Wang, Josiah Levi Davidson, Simerdeep Kaur, Andres Dextre, Mohsen Ranjbaran, Mohamed Kamel, Shreya Milind Athalye, Mohit S. Verma
Abstract
Paper-based biosensors are microfluidic analytical devices used for the detection of biochemical substances. The unique properties of paper-based biosensors, including low cost, portability, disposability, and ease of use, make them an excellent tool for point-of-care testing. Among all analyte detection methods, nucleic acid-based pathogen detection offers versatility due to the ease of nucleic acid synthesis. In a point-of-care testing context, the combination of nucleic acid detection and a paper-based platform allows for accurate detection. This review offers an overview of contemporary paper-based biosensors for detecting nucleic acids from pathogens. The methods and limitations of implementing an integrated portable paper-based platform are discussed. The review concludes with potential directions for future research in the development of paper-based biosensors.