Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR
Yanxiong Pan, Hui Li, Qiaobin Li, Mary Lenertz, I. Schuster, Drew Jordahl, Xiao Zhu, Bingcan Chen, Zhongyu Yang
Abstract
Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of enzymes in MOFs/COFs using site-directed spin labeling and electron paramagnetic resonance spectroscopy. The protocol is demonstrated using lysozyme and can be generalized to other enzymes. For complete information on the generation and use of this protocol, please refer to Pan et al. (2021a).
Topics & Concepts
Electron paramagnetic resonanceSite-directed spin labelingElectron paramagnetic resonance spectroscopyCovalent bondChemistryEnzymeProtocol (science)Orientation (vector space)Materials scienceNanotechnologyNuclear magnetic resonanceBiochemistryPhysicsOrganic chemistryMedicineGeometryPathologyAlternative medicineMathematicsLanthanide and Transition Metal ComplexesElectron Spin Resonance StudiesMetal-Organic Frameworks: Synthesis and Applications