Competitiveness for Nodule Colonization in Sinorhizobium meliloti: Combined <i>In Vitro</i> -Tagged Strain Competition and Genome-Wide Association Analysis
Agnese Bellabarba, Giovanni Bacci, Francesca Decorosi, Erki Aun, Elisa Azzarello, Maido Remm, Luciana Giovannetti, Carlo Viti, Alessio Mengoni, Francesco Pini
Abstract
-mer-based GWAS approach can efficiently identify the competition determinants of a panel of strains previously analyzed for their plant tissue occupancy using double fluorescent labeling. The reported strategy will be useful for detailed studies on the genomic aspects of the evolution of bacterial symbiosis and for an extensive evaluation of rhizobial inoculants.
Topics & Concepts
BiologySinorhizobium melilotiRhizobiaGenomeSymbiosisSinorhizobiumGeneticsGeneLotus japonicusStrain (injury)MicrobiologyBacteriaAnatomyLegume Nitrogen Fixing SymbiosisNematode management and characterization studiesAgronomic Practices and Intercropping Systems