Indirectly stimulation of DCs by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model based on RNA-seq
Xiaomeng Ding, Qiang Yu, Kunyou Hou, Xiaoyi Hu, Yudan Wang, Yi Chen, Jianhua Xie, Shaoping Nie, Mingyong Xie
Abstract
Our previous study has demonstrated that Ganoderma atrum polysaccharide (PSG-1) activated the immune response of dendritic cells (DCs) by direct contacting with surface receptors. However, as a kind of macromolecule compound, PSG-1 cannot directly stimulate DCs via passing through the intestine epithelial cells. To investigate the role of PSG-1 in the interaction between the intestinal epithelium and its internal immune cells, the intestinal-like Caco-2/DCs co-culture model was established, then all transcripts of DCs with or without treatment of PSG-1 were globally screened by RNA-seq. According to the RNA-seq analysis, cytokine receptor interaction, NF-κB and MAPK signaling pathway were three important signal pathways annotate to the environmental information processing. Moreover, cytokine and related proteins expression were in DCs indirectly stimulated by PSG-1 were raised. These above findings indicated that PSG-1 indirectly affected the immune function of DCs in intestinal-like Caco-2/DCs co-culture model, in which NF-κB and MAPK pathway played essential roles.