Screening of H2S donors with a red emission mitochondria-targetable fluorescent probe: Toward discovering a new therapeutic strategy for Parkinson's disease
Ke Wu, Xumei Wang, Xumei Wang, Lili Gong, Xinyuan Zhai, Kai Wang, Xiao Qiu, Hao Zhang, Zhixin Tang, Haiqiang Jiang, Xiaoming Wang, Xiaoming Wang
Abstract
Parkinson's disease (PD) is a neurodegenerative disorder caused by various factors such as neuroinflammation , oxidative stress , mitochondrial dysfunction, and neuronal apoptosis . Recent studies have shown that H 2 S supplementation reverses neuronal loss and mitigates motor deficits in PD patients through anti-inflammatory, antioxidant , improved mitochondrial function and proautophagic. Therefore, the discovery and use of H 2 S donors may be an exciting and intriguing strategy for the treatment of PD . Herein, we report a red emission mitochondria-targetable fluorescent probe , Rho-H 2 S , which can specifically and sensitively detect H 2 S with a limit of detection of 62.5 nM. Bioimaging experiments have shown that the probe has excellent mitochondrial targeting and good imaging capabilities for the detection of exogenous and endogenous H 2 S in cells. More importantly, based on the Rho-H 2 S probe, we first confirmed the sulforaphane (SFN) among 15 glucosinolate and isothiocyanate compounds from cruciferous vegetables with an outstanding ability to release H 2 S and we further proved that SFN could alleviate the symptoms of PD in vivo . All results demonstrate that Rho-H 2 S could be an effective tool for screening H 2 S donors and can contribute to the development of new therapeutic strategies for PD.