Reverse Transcription-Loop-Mediated Isothermal Amplification-CRISPR-Cas13a Technology as a Promising Diagnostic Tool for SARS-CoV-2
Concha Ortiz-Cartagena, Laura Fernández-García, Lucía Blasco, Olga Pacios, Inés Bleriot, María López, Rafael Cantón, María Tomás
Abstract
The coronavirus disease 2019 (COVID-19) crisis has driven the development of innovative molecular diagnosis methods, including CRISPR-Cas technology. In this work, we performed a protocol, working with RNA extraction kit-free samples and using RT-LAMP-CRISPR-Cas13a technology; our results place this method at the forefront of rapid and specific diagnostic methods for COVID-19 due to the high specificity (100%), sensitivity (83%), PPVs (100%), and NPVs (81% for high viral loads) obtained with clinical samples.
Topics & Concepts
Loop-mediated isothermal amplificationCRISPRRNA extractionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)RNAReverse Transcription Loop-mediated Isothermal AmplificationContext (archaeology)Coronavirus disease 2019 (COVID-19)Computational biologyReverse transcriptaseVirologyBiologyMedicineGeneDNAGeneticsDiseaseInfectious disease (medical specialty)PathologyPaleontologyCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical Detection