Reactivity-Based Screening for Citrulline-Containing Natural Products Reveals a Family of Bacterial Peptidyl Arginine Deiminases
Lonnie A. Harris, Patricia M. B. Saint‐Vincent, Xiaorui Guo, Graham A. Hudson, Adam J. DiCaprio, Lingyang Zhu, Douglas A. Mitchell
Abstract
-citrulassin producer resulted in the production of the deiminated analog, confirming PAD involvement in Arg deimination. The PADs were then bioinformatically surveyed to provide a deeper understanding of their taxonomic distribution and genomic contexts and to facilitate future studies that will evaluate any additional biochemical roles for the superfamily.
Topics & Concepts
CitrullinationPeptideBiochemistryBiologyGeneArginineGeneticsGene isoformProteolysisCitrullineComputational biologyChemistryAmino acidEnzymeAntimicrobial Peptides and ActivitiesMicrobial Natural Products and BiosynthesisBiochemical and Structural Characterization