<i>Escherichia coli</i> with a 57-codon genetic code
Wesley E. Robertson, Fabian B. H. Rehm, Martin Spinck, R. R. Schumann, Rongzhen Tian, Wei Liu, Yangqi Gu, Askar A. Kleefeldt, Cicely F. Day, Kim C. Liu, Yonka Christova, Jérôme F. Zürcher, Franz L. Böge, Jakob Birnbaum, Linda van Bijsterveldt, Jason W. Chin
Abstract
The near-universal genetic code uses 64 codons to encode the 20 canonical amino acids and protein synthesis. Here, we designed and generated Escherichia coli with a 4-megabase synthetic genome in which we replaced known occurrences of six sense codons and a stop codon with synonymous codons. The resulting organism, Syn57, uses 55 codons to encode the 20 canonical amino acids.
Topics & Concepts
Genetic codeEscherichia coliENCODEGeneticsCodon usage biasAmino acidBiologyGenomeStop codonGeneComputational biologyRNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyCRISPR and Genetic Engineering