Defining genome-wide CRISPR–Cas genome-editing nuclease activity with GUIDE-seq

Nikolay Malinin, GaHyun Lee, Cícera R. Lazzarotto, Yichao Li, Zongli Zheng, Nathalie T. Nguyen, Matthew Liebers, Ved V. Topkar, A. John Iafrate, Long P. Le, Martin J. Aryee, J. Keith Joung, Shengdar Q. Tsai