Prediction of Transcription Factors Binding Events Based on Epigenetic Modifications in Different Human Cells
Yan Huang, Dianshuang Zhou, Yihan Wang, Xingda Zhang, Mu Su, Cong Wang, Zhongyi Sun, Qinghua Jiang, Baoqing Sun, Yan Zhang
Abstract
Aim: We aim to predict transcription factor (TF) binding events from knowledge of gene expression and epigenetic modifications. Materials & methods: TF-binding events based on the Encode project and The Cancer Genome Atlas data were analyzed by the random forest method. Results: We showed the high performance of TF-binding predictive models in GM12878, HeLa, HepG2 and K562 cell lines and applied them to other cell lines and tissues. The genes bound by the top TFs (MAX and MAZ) were significantly associated with cancer-related processes such as cell proliferation and DNA repair. Conclusion: We successfully constructed TF-binding predictive models in cell lines and applied them in tissues.