Light-up split aptamers: binding thermodynamics and kinetics for sensing
Yichen Zhao, Nikesh Patel, Pei Huan Sun, Karen Faulds, Duncan Graham, Juewen Liu
Abstract
. Temperature-dependent fluorescence measurements confirmed the lower thermostability of split aptamers. Isothermal titration calorimetry indicated that split aptamer binding can release more heat but have an even larger entropy loss. Finally, a colorimetric biosensor using gold nanoparticles was designed by pre-assembling two thiolated aptamer halves, which can then link gold nanoparticles to give a red-to-blue color change.
Topics & Concepts
AptamerIsothermal titration calorimetryDissociation constantChemistryThermostabilityFluorescenceBiosensorSystematic evolution of ligands by exponential enrichmentNanoparticleNanotechnologyKineticsCombinatorial chemistryBiophysicsMaterials scienceRNABiochemistryBiologyMolecular biologyPhysicsOpticsEnzymeReceptorGeneQuantum mechanicsAdvanced biosensing and bioanalysis techniquesMolecular Junctions and NanostructuresBiosensors and Analytical Detection