Construction and validation of m <sup>6</sup> A RNA methylation regulators associated prognostic model for gastrointestinal cancer
Yandong Miao, Bin Su, Xiaolong Tang, Jiangtao Wang, Wuxia Quan, Yonggang Chen, Denghai Mi
Abstract
Abstract N6‐methyladenosine (m 6 A) RNA methylation is correlated with carcinogenesis and dynamically possessed through the m 6 A RNA methylation regulators. This paper aimed to explore 13 m 6 A RNA methylation regulators' role in gastrointestinal cancer (GIC) and determine the risk model and prognosis value of m 6 A RNA methylation regulators in GIC. We used several bioinformatics methods to identify the differential expression of m 6 A RNA methylation regulators in GIC, constructed a prognostic model, and carried out functional enrichment analysis. Eleven of 13 m 6 A RNA methylation regulators were differentially expressed in different clinicopathological characteristics of GIC, and m 6 A RNA methylation regulators were nearly associated with GIC. We constructed a risk model based on five m 6 A RNA methylation regulators (METTL3, FTO, YTHDF1, ZC3H13, and WTAP); the risk score is an independent prognosis biomarker. Moreover, the five m 6 A RNA methylation regulators can also forecast the 1‐, 3‐ and 5‐year overall survival through a nomogram. Furthermore, four hallmarks of oxidative phosphorylation, glycolysis, fatty acid metabolism, and cholesterol homoeostasis gene sets were significantly enriched in GIC. m 6 A RNA methylation regulators were related to the malignant clinicopathological characteristics of GIC and may be used for prognostic stratification and development of therapeutic strategies.