Interplay Between Plasma Membrane Lipid Alteration, Oxidative Stress and Calcium-Based Mechanism for Extracellular Vesicle Biogenesis From Erythrocytes During Blood Storage
Anne‐Sophie Cloos, Marine Ghodsi, Amaury Stommen, Juliette Vanderroost, Nicolas Dauguet, Hélène Pollet, Ludovic D’Auria, Éric Mignolet, Yvan Larondelle, Romano Terrasi, Giulio G. Muccioli, Patrick Van Deŕ Smissen, Donatienne Tyteca
Abstract
/EDTA tubes supplemented with glucose to longer preserve the ATP content, we better defined the sequence of events. Altogether, we showed that EV shedding from lipid domains only represents part of the global vesiculation mechanistics, for which we propose four successive events (cholesterol domain decrease, oxidative stress, sphingomyelin/sphingomyelinase/ceramide/calcium alteration and phosphatidylserine exposure).
Topics & Concepts
SphingomyelinAcid sphingomyelinaseCeramideChemistryPhosphatidylserineCholesterolLipid peroxidationBiochemistryOxidative stressMembrane lipidsVesicleRed blood cellCell biologyBiologyMembraneApoptosisPhospholipidErythrocyte Function and PathophysiologyExtracellular vesicles in diseaseBlood properties and coagulation