CRISPR-Sunspot: Imaging of endogenous low-abundance RNA at the single-molecule level in live cells
Ninghe Sun, Danyang Chen, Lu-Peng Ye, Gang Sheng, Junjie Gong, Baohui Chen, Ying‐Mei Lu, Feng Han
Abstract
Our findings suggest that CRISPR-Sunspot is a novel applicable imaging tool for visualizing the distributions of low-abundance mRNAs in cells. This study provides a novel strategy to unravel the molecular mechanisms of diseases caused by aberrant mRNA molecules.
Topics & Concepts
CRISPRSubgenomic mRNAMessenger RNABiologyCell biologyGreen fluorescent proteinRNALive cell imagingComputational biologyCellGeneGeneticsCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsRNA Research and Splicing