pH-dependent selective separation of acidic and basic proteins using quaternary ammoniation functionalized cysteine-zwitterionic stationary phase with RPLC/IEC mixed-mode chromatography
Jianzhong Wang, Jianshan Wang, Xiaohui Ning, Jiawei Liu, Hongjun Xia, Guangping Wan, Quan Bai
Topics & Concepts
ChemistrySelectivityAcetonitrileCysteineChromatographyPhase (matter)Hydrophilic interaction chromatographySilica gelChemical modificationLigand (biochemistry)Inorganic chemistryOrganic chemistryHigh-performance liquid chromatographyPolymer chemistryCatalysisReceptorEnzymeBiochemistryAnalytical Chemistry and ChromatographyProtein purification and stabilityMicrofluidic and Capillary Electrophoresis Applications