Structural basis of transcription-translation coupling
Chengyuan Wang, Vadim Molodtsov, Emre Firlar, Jason T. Kaelber, Gregor Blaha, Min Su, Richard H. Ebright
Abstract
transcription-translation complexes (TTCs) containing different-length mRNA spacers between RNAP and the ribosome active-center P site. Structures of TTCs containing short spacers show a state incompatible with NusG bridging and NusA binding (TTC-A, previously termed "expressome"). Structures of TTCs containing longer spacers reveal a new state compatible with NusG bridging and NusA binding (TTC-B) and reveal how NusG bridges and NusA binds. We propose that TTC-B mediates NusG- and NusA-dependent transcription-translation coupling.
Topics & Concepts
RibosomeRNA polymeraseTranscription (linguistics)Bacterial transcriptionMessenger RNABiologyTranslation (biology)Cell biologyRNABiophysicsChemistryComputational biologyGeneticsGeneLinguisticsPhilosophyRNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyRNA modifications and cancer