Extracellular <scp>S100B</scp> inhibits A‐type voltage‐gated potassium currents and increases L‐type voltage‐gated calcium channel activity in dopaminergic neurons
Eric A. Bancroft, Martha De La Mora, Gauri Pandey, Sara M. Zarate, Rahul Srinivasan
Abstract
Abstract Parkinson's disease (PD) is associated with an increase in secreted S100B within the midbrain and cerebrospinal fluid. In addition, S100B overexpression in mice accelerates the loss of substantia nigra pars compacta dopaminergic (DA) neurons, suggesting a role for this protein in PD pathogenesis. We found that in the mouse SNc, S100B labeled astrocytic processes completely envelop the somata of tyrosine hydroxylase (TH) expressing DA neurons only in male mice. These data suggest that an increase in S100B secretion by astrocytes within the midbrain could play a role in DA dysfunction during early PD. We therefore asked if acute exposure to extracellular S100B alters the activity of identified TH expressing DA neurons in primary mouse midbrain cultures. Acute exposure to 50 pM S100B specifically inhibited A‐type voltage‐gated potassium currents in TH + , but not TH − neurons. This was accompanied by ~2‐fold increases in the frequency of both intrinsic firing, as well as L‐type voltage‐gated calcium channel (VGCC)‐mediated calcium fluxes only in TH + neurons. Further, exposure to 100 μM 4‐aminopyridine (4‐AP), an A‐type voltage‐gated potassium channel inhibitor, mimicked the S100B mediated increase in intrinsic firing and L‐type VGCC‐mediated calcium fluxes in TH + neurons. Taken together, our finding that extracellular S100B alters the activity of native DA neurons via an inhibition of A‐type voltage‐gated potassium channels has important implications for understanding the pathophysiology of early PD.