High-throughput cryo-EM structure determination of amyloids
Sofia Lövestam, Sjors H. W. Scheres
Abstract
assembly conditions. To this end, we describe a new filament picking algorithm based on the Topaz approach, and we outline image processing strategies in Relion that enable atomic structure determination of amyloids within days.
Topics & Concepts
Protein filamentThroughputComputer scienceCryo-electron microscopyNanotechnologyMaterials scienceBiologyBiophysicsComposite materialWirelessTelecommunicationsAdvanced Electron Microscopy Techniques and ApplicationsEnzyme Structure and FunctionProtein Structure and Dynamics