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Efficient NADH Regeneration without Electron Mediator toward Enzymatic CO<sub>2</sub> Reduction Enabled by a Lawn-like TP-COFs/Ti<sub>3</sub>C<sub>2</sub><i>T</i><sub><i>x</i></sub> (MXene) Photocatalyst

Ping Wei, Jinfeng Dong, Xingyue Gao, Lekai Chang, Zhipeng Huang, Hailong Zheng, Simon Ming‐Yuen Lee, Wen‐Yong Lou, Chao Peng

2024ACS Sustainable Chemistry & Engineering23 citationsDOI

Abstract

Photocatalytic nicotinamide adenine dinucleotide (NADH) regeneration coupled with enzymatic CO 2 reduction holds significance for global sustainable development. In this work, acrylonitrile-linked covalent organic frameworks (COFs) loaded on NH 2 –Ti 3 C 2 T x (MXene) forms a lawn-like TP-COFs/Ti 3 C 2 T x (TCM) photocatalyst for NADH regeneration. The optimum photocatalyst TCM-15% achieves a 95% NADH regeneration yield within 30 min. More importantly, the TCM-15% obtains 46% NADH regeneration yield without an electron mediator ([Cp*Rh(bpy)(H 2 O)] 2+ ), even higher than that of the pure TP-COFs with an electron mediator (42%). In the presence and absence of an electron mediator, the NADH regeneration TOF values of TCM-15% were 3.69 and 1.42 h –1, respectively, which were 4.05 and 4.90 times higher than those of pure TP-COFs. Subsequently, in the photoenzymatic catalytic cascade system, the formate formation rates reached 2380 and 543 μmol g –1 h –1, with and without an electron mediator, respectively. This work has laid a great foundation for achieving efficient NADH regeneration and cascade enzyme catalysis without an electron mediator.

Topics & Concepts

MediatorChemistryRegeneration (biology)EnzymeCatalysisElectron donorNAD+ kinaseAcrylonitrilePhotochemistryBiochemistryBiologyOrganic chemistryCell biologyPolymerCopolymerAdvanced Photocatalysis TechniquesCovalent Organic Framework ApplicationsMXene and MAX Phase Materials