Carbon Nanodot–Neutral Red-Based Photometric and Fluorescence Sensing for Trace Detection of Nitrite in Water and Soil Using Smartphone
Priyanka Das, Sritam Biswas, Satya Sundar Bhattacharya, Pabitra Nath
Abstract
Nitrite level estimation in soil and water bodies is critical to monitor the ecosystem of our environment and agricultural yield. Herein, we report a carbon nanodots (C-dots) and neutral red (NR)-based photometric and fluorescence mode sensing scheme using which trace detection of nitrite in water and soil has been done. The proposed scheme utilizes a handheld compact sensing platform developed on a smartphone. For the present sensing studies, the C-dots and NR act as donors and acceptors, respectively, in the frequency resonance energy transfer (FRET) process. The presence of nitrite in the C-dot–NR mixture affects the FRET process, which causes a decrease in the absorption wavelength conditions at 560 nm while increasing the fluorescence intensity at 563 nm upon excitation with a source of wavelength 375 nm. These variations have been correlated to quantify the concentration of the nitrite level in the medium. With the designed sensing system, variations of nitrite concentration from 0.1 to 3.0 μg/mL have been measured with a high degree of accuracy. The designed sensor has been implemented to estimate the nitrite level of infield water and soil samples, and the experimental results are compared with the laboratory standard tools. With the advantages of being low-cost, field portable, and relatively convenient to handle, it is anticipated that the proposed smartphone sensor could emerge as a potential avenue for onsite assessment of other parameters of water and soil as well.