High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails
Brian R. Shy, Vivasvan S. Vykunta, Alvin Ha, Alexis Talbot, Theodore L. Roth, David N. Nguyen, Wolfgang Pfeifer, Yan Yi Chen, Franziska Blaeschke, Eric Shifrut, Shane Vedova, Murad R. Mamedov, Jing-Yi Chung, Hong Li, Ruby Yu, David Wu, Jeffrey L. Wolf, Thomas Martin, Carlos E. Castro, Lumeng Ye, Jonathan H. Esensten, Justin Eyquem, Alexander Marson
Topics & Concepts
CRISPRGenome editingDNACas9GeneGenomeComputational biologyPlasmidTemplateBiologyHomology directed repairTransgeneChemistryGeneticsMolecular biologyDNA repairNanotechnologyNucleotide excision repairMaterials scienceCRISPR and Genetic EngineeringCAR-T cell therapy researchVirus-based gene therapy research