Lysin LysMK34 of<i>Acinetobacter baumannii</i>Bacteriophage PMK34 Has a Turgor Pressure-Dependent Intrinsic Antibacterial Activity and Reverts Colistin Resistance
Karim Abdelkader, Diana Gutiérrez, Dennis Grimon, Patricia Ruas‐Madiedo, Cédric Lood, Rob Lavigne, Amal Safaan, Ahmed S. Khairalla, Yasser Gaber, Tarek Dishisha, Yves Briers
Abstract
A. baumannii is one of the most challenging pathogens for which development of new and effective antimicrobials is urgently needed. Colistin is a last-resort antibiotic, and even colistin-resistant A. baumannii strains exist. Here, we present a lysin that sensitizes A. baumannii for colistin and can revert colistin resistance to colistin susceptibility. The lysin also shows a strong, turgor pressure-dependent intrinsic antibacterial activity, providing new insights in the mode of action of lysins with intrinsic activity against Gram-negative bacteria.
Topics & Concepts
Acinetobacter baumanniiBacteriophageLysinMicrobiologyColistinAcinetobacterBiologyTurgor pressurePolymyxinAntimicrobialBacteriaAntibioticsEscherichia coliGeneticsBotanyPseudomonas aeruginosaGeneAntibiotic Resistance in BacteriaBacteriophages and microbial interactionsBacterial Genetics and Biotechnology